Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

OKT 8 antibody


  • Please log in to reply
3 replies to this topic

#1 shilpeejnc

shilpeejnc

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 22 May 2011 - 08:46 AM

I am trying to purify OKT8 antibody from mouse ascites. I tried in balb/c mouse but ascites didn't develop. can any body trouble shoot me. how it can be done? also how OKT8 hybridoma has been generated? what strategy has been used to confirm them?

thanks

#2 Chelo

Chelo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 94 posts
3
Neutral

Posted 22 May 2011 - 09:52 AM

Hi!
You must be sure your hybridoma is syngeneic with the Balb/c mouse strain. In other words, lymphocytes and myeloma cells used for the fusion have to be Balb/c cells as well. Otherwise, the hybridoma cells might be rejected by the mouse immune system.
If your hybridoma is actually syngeneic, make sure you inject pristane the mice.
I hope this helps.

#3 shilpeejnc

shilpeejnc

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 23 May 2011 - 04:24 AM

pristane i have used. Mouse is not syngenic, but its not even rejected . there is no immune response symptoms


Hi!
You must be sure your hybridoma is syngeneic with the Balb/c mouse strain. In other words, lymphocytes and myeloma cells used for the fusion have to be Balb/c cells as well. Otherwise, the hybridoma cells might be rejected by the mouse immune system.
If your hybridoma is actually syngeneic, make sure you inject pristane the mice.
I hope this helps.



#4 Chelo

Chelo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 94 posts
3
Neutral

Posted 23 May 2011 - 06:03 AM

pristane i have used. Mouse is not syngenic, but its not even rejected . there is no immune response symptoms



Hi!
You must be sure your hybridoma is syngeneic with the Balb/c mouse strain. In other words, lymphocytes and myeloma cells used for the fusion have to be Balb/c cells as well. Otherwise, the hybridoma cells might be rejected by the mouse immune system.
If your hybridoma is actually syngeneic, make sure you inject pristane the mice.
I hope this helps.


Hi again!

I am not sure to follow you arguments. If cells are rejected, you won´t see any "symptoms" since we are talking about individual cells, not an organ. In other words, your hybridoma cells must be killed and you won´t be able to notice that. Better change mouse strain. Alternatively, try higher number of injected cells. We try with 1 million cells for isogenic injection. You might want to try 10-100 times that number for non-related mice.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.