SPONTAEOUS DEATH OF CELLS in culture
Posted 22 May 2011 - 04:06 AM
Posted 22 May 2011 - 04:17 AM
Edited by Biouday, 22 May 2011 - 04:18 AM.
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Posted 22 May 2011 - 05:09 AM
It is not clear what you treated with. It always suggest to check the cytotoxity of chemical which you treating before doing the actually experiment. i think peeling was because of toxic effect of the treatment.
but the peeling occurred before treatment
Posted 22 May 2011 - 07:11 AM
Posted 22 May 2011 - 10:36 PM
How confluent are they? I've not worked with B16 cells very much, but I did find that they did not like being 100% confluent and started to lift if I allowed them to get too confluent. Maybe try plating fewer cells into your 24 well trays?
i dont think this can be the reason because peeling cells stil looks round and my cells looks dead
Posted 23 May 2011 - 05:10 PM
Did you recently change supplier of FCS/FBS or plates?
Posted 24 May 2011 - 12:08 AM
i did not change a thing in the lab and the cells grow fine in flasks, i see this phenomena just in wells (24/96)
Posted 24 May 2011 - 06:26 PM
If it is only a problem in the plates, try a new batch and check your seeding densities, which are a common cause of cells not attaching properly.
Posted 20 September 2011 - 08:47 AM