Unexpected expression of my protein in HepG2
Posted 21 May 2011 - 08:30 PM
Posted 22 May 2011 - 05:57 PM
Posted 22 May 2011 - 08:17 PM
Is the antibody you are using a new one for the same protein? How have you confirmed that this is the protein you think it is on your westerns (e.g. by peptide competition)?
Yes, I have confirmed it by treating the cells with a specific inhibitor of the protein..the expression level dropped. Plus, I run a positive control which showed up at the same molecular weight. No change has been made to the antibody used. Different aliquots of the same Ab stock.
Posted 23 May 2011 - 05:07 PM
Absence of evidence is not evidence of absence.