can't get bands in Western Blot
Posted 21 May 2011 - 08:03 PM
Posted 22 May 2011 - 02:29 AM
- hobglobin, personal comment about my beauteous photo......
Posted 22 May 2011 - 04:34 AM
Posted 22 May 2011 - 04:36 AM
Posted 22 May 2011 - 07:53 AM
Also, both the % of PAGE gel and the Bis-Acryl:Acrylamide ratio is important.
Maybe your PAGE gel has higher B:A which caused the separation not good enough.
You have to play with both for an optimum separation.
I not sure if your lysis buffer consist of protease inhibitors. It might be a good idea if you can add some in it.
IF all fail, refer to this article:
Try to contact the authors and see their opinion. They had done extensive and comprehensive work in both protein you mentioned.
In the mean time, keep us update about your troubleshooting and perhaps others can be benefited from your findings.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434