Here are my questions:
1. The abbreviation RBC buffer mean Red Blood Cells buffer, right? Why do we use it? I know that due to some pH changes it promotes retain of RNA only on the gDNA Eliminator spin column, but it that correct? Why it is called RBC then?
2. What does mean the abbreviation RPE and why do we use this buffer? For me it sound like RNA Precipitating Elution Buffer - it keeps the RNA precipitated on the RNEasy spin column but allows discard of the chemical contaminants, and then we elute the pure (more or less) RNA in RNAse free water. Is that correct?
I will appreciate any answer.
Edited by Nephrit, 21 May 2011 - 04:51 AM.