Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Caspase 3 inhibitor (Z-DEVD-FMK)

  • Please log in to reply
No replies to this topic

#1 barteee



  • Members
  • Pip
  • 2 posts

Posted 20 May 2011 - 08:52 AM

I'm studying a virus which induces apoptosis in multiple myeloma cells (as measured by loss of viability as well as cleavage of Casp3 and PARP). I tried to inhibit this apoptosis with a caspase 3 inhibitor Z-DEVD-FMK at 100uM (the highest concentration recommended by the company), preincubation of cells with drug for 1 hour followed by virus infection, and analysis of apoptosis using CASP3 and PARP cleavage at 24 hours. I saw virtually no reduction in cells death and only a very small reduction in cleavage of PARP using the drug.

Anyone ever use this drug before? Does the drug just not inhibit caspase-3 100% or is there non-caspase-3 mediated cleavage of PARP?


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.