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Musca domestica gDNA isolation


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#1 CHODAR

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Posted 19 May 2011 - 08:55 AM

Hi people,

I wan to ask if some of you have experience with gDNA extracction from housfly individuals. I use a protocol described in Moreira et.al. (Insect Science, 4:2. 2004) All is going well until I run the gDNA on agarose gel (1%). Togheter with the band over 10kb, I see another band close to 1kb (see pic).

Someone see something like that in gDNA electrophoresis? Partial digesting of gDNA? Supercoiled gDNA? Contamination?

Thanks for the help.

Christian.

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  • mdomestica.gDNA.jpg


#2 hobglobin

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Posted 19 May 2011 - 10:56 AM

RNA? There can be surprisingly much and large in insects. Add RNAse and see if the band is still there.
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#3 CHODAR

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Posted 19 May 2011 - 11:48 AM

RNA? There can be surprisingly much and large in insects. Add RNAse and see if the band is still there.


Could be...in fact, im treating right now..

I keep you inform. Thanks!

Christian.

#4 CHODAR

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Posted 19 May 2011 - 03:05 PM

RNA? There can be surprisingly much and large in insects. Add RNAse and see if the band is still there.


Well..problem result.
Thanks for your concern hobglobin.

Christian.

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  • mdomestica.gDNA.19.05.11.jpg





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