Musca domestica gDNA isolation
Posted 19 May 2011 - 08:55 AM
I wan to ask if some of you have experience with gDNA extracction from housfly individuals. I use a protocol described in Moreira et.al. (Insect Science, 4:2. 2004) All is going well until I run the gDNA on agarose gel (1%). Togheter with the band over 10kb, I see another band close to 1kb (see pic).
Someone see something like that in gDNA electrophoresis? Partial digesting of gDNA? Supercoiled gDNA? Contamination?
Thanks for the help.
Posted 19 May 2011 - 10:56 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 19 May 2011 - 11:48 AM
Could be...in fact, im treating right now..
I keep you inform. Thanks!
Posted 19 May 2011 - 03:05 PM
Thanks for your concern hobglobin.