3 replies to this topic

[CHODAR]

Hi people,

I wan to ask if some of you have experience with gDNA extracction from housfly individuals. I use a protocol described in Moreira et.al. (Insect Science, 4:2. 2004) All is going well until I run the gDNA on agarose gel (1%). Togheter with the band over 10kb, I see another band close to 1kb (see pic).

Someone see something like that in gDNA electrophoresis? Partial digesting of gDNA? Supercoiled gDNA? Contamination?

Thanks for the help.

Christian.

Attached Thumbnails

• 

[hobglobin]

RNA? There can be surprisingly much and large in insects. Add RNAse and see if the band is still there.

[CHODAR]

hobglobin, on 19 May 2011 - 10:56 AM, said:

RNA? There can be surprisingly much and large in insects. Add RNAse and see if the band is still there.

Could be...in fact, im treating right now..

I keep you inform. Thanks!

Christian.

[CHODAR]

hobglobin, on 19 May 2011 - 10:56 AM, said:

RNA? There can be surprisingly much and large in insects. Add RNAse and see if the band is still there.

Well..problem result.
Thanks for your concern hobglobin.

Christian.

Attached Thumbnails

•