This is my PCR products (from cDNA) using seed from tomato. Lane 1-6 are cDNA synthesized from RNA without any DNA removal while lane 7-10 are cDNA synthesized from RNA treated with DNase removal. Line 1 using 1st primer (with intron, PCR product= 536bp and without intron, PCR product=346bp). lane 3 using primer from the intron sequence (312bp). Lane 4 is housekeeping gene (UBQ=302bp). Lane 5 and 6 are other housekeeping genes. Same goes to the lane 7-10 except using cDNA with DNAse removal.
So, why did I got PCR product (lane 1) where the intron is still there and no PCR product on lane 7 after DNA removal?????
Edited by seed, 19 May 2011 - 07:19 AM.