Hi again everyone,
I am doing a microsatellite analysis & have to run Urea PAGE for my PCR products, my product size range is 116 bp to 300 bp, for what time should i pre run & run the gel, what is the criteria for deciding the run time & pre run time ? Is there any effect of size of the plate on resolution power, i am having a bio rad mini sequencer that is too bulky to handle & requires too much buffer & chemicals, alternatively i am also having a smaller vertical PAGE assemble of about 8inches height, which one should i use ? also wanted to know the concentration of silver nitrate during silver staining procedure.
Thnx
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