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Soft Agar Assay


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#1 anonymous

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Posted 08 November 2001 - 10:00 PM

Does anybody know to do the soft agar assay with mda-mb-231 cells?While doing the assay the agar always congeals when mixed with the media.I use DMEM. Can anybody suggest a detailed protocol with the conc. of stock agar/agarose used for preparing the base gel and the top gel.

ThanksKedar


#2 anonymous

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Posted 10 November 2001 - 10:00 PM

Hi!For base agar it is usually 3% and top agar it is 1%.try this. It may wotk.all the best

#3 anonymous

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Posted 10 November 2001 - 10:00 PM

Hi!For base agar it is usually 3% and top agar it is 1%.try this. It may wotk.all the best




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