Soft Agar Assay
2 replies to this topic
Posted 08 November 2001 - 10:00 PM
Does anybody know to do the soft agar assay with mda-mb-231 cells?While doing the assay the agar always congeals when mixed with the media.I use DMEM. Can anybody suggest a detailed protocol with the conc. of stock agar/agarose used for preparing the base gel and the top gel.