pcr amplify an insert in plasmid?
Posted 18 May 2011 - 02:57 AM
Posted 18 May 2011 - 08:57 PM
If you need to look for the gene on the plasmid specifically, if for example the gene is also present in the cell natively, the anchor the primers in the plasmid sequence somewhere, or look for a plasmid specific gene such as Ampr or B-gal.
Posted 23 May 2011 - 02:56 AM
I never trust anything that can't be doubted.