Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA precipitation


  • Please log in to reply
1 reply to this topic

#1 Myco Rax

Myco Rax

    member

  • Active Members
  • Pip
  • 19 posts
1
Neutral

Posted 17 May 2011 - 11:34 PM

Dear All,
I have to purifiy RNA from an immunoprecipitated (RNA immunoprecipitation)sample.
can anyone suggest me how to precipitate RNA using Sodium acetate (conc??) and Isopropanol (volume??) directly without any preceding steps involving trizol or phenol?
Thanks in Advance.

#2 lsek

lsek

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 52 posts
1
Neutral

Posted 23 May 2011 - 08:22 AM

Hi,

Try selective precipitation of RNA if you want to by-pass organic extraction (e.g. Trizol or phenol). This is accomplished by using LiCl with final concentration of 2 to 3M. You can wash the resultant pellet with 3M LiCl twice before reconstituting the RNA pellet in DEPC treated water.

An extra ethanol precipitation step can be incorporated after LiCl preciptation (if needed).

Bests,

...-...

===><===





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.