ISH - ethanol dehydration of whole mount embryos
Posted 17 May 2011 - 09:55 AM
Can someone please tell me why NBT/BCIP signal bleaches out/vanishes after I dehydrate my embryos using ethanol?
How can I prevent this? Is dehydration even necessary?
Grateful for any advice on this.
Posted 19 May 2011 - 06:56 AM
genius does what it must
i do what i get paid to do
Posted 19 May 2011 - 08:47 AM