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[katcvet]

I want to send RNA (in the form of total RNA) for deep sequencing using Illimuna. My RNA is from clinical material so I'm concerned re: degradation. I intend to check the extent of degradation prior to sending the sample for sequencing. However if degradation is a big problem I can't do a great deal about it! I've read that if my RNA is degraded it will result in a 3' bias when it comes to sequencing - but what does this actually mean, and why does it happen?! Thanks