I am currently undertaking a PhD work in the immunology barnch of the GIMAP laboratory in the St Etienne school of Medicine (France). My work is under the direction of Pr. C GENIN and Dr. O GARRAUD.
I am looking for a precise expérimental protocol for human B cell and PBMC culture (conditions, type of plate, cell density, reagents…) allowing me to decrease the baseline production of human Ig during cell culture – This Ig product is measured by ELISA.
I would like also to optimize culture conditions to increase Ig production.
I would be very grateful if you gave me your precious help.