Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Colony PCR

  • Please log in to reply
1 reply to this topic

#1 AGM1



  • Active Members
  • Pip
  • 9 posts

Posted 16 May 2011 - 06:46 PM


I have created a genomic library that contains environmental insert DNA, and the host is E.coli. The library is made of fosmids, and the copy number is kept at one. I was planning on using colony pcr to screen the inserts present in the library. I was wondering if its possible to use
gram-positive pcr primers to screen the library, so as to avoid the host E.coli DNA, and still be able to screen for a large number of bacterial 16S genes. If anyone with expierience in these techniques could shed some light, it would be appreciated

Thank you

#2 BioMiha



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 237 posts

Posted 17 May 2011 - 09:01 PM

Why don't you use fosmid specific primers. That's how it is usually done. That way you don't need to worry about amplifying the host gDNA.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.