I have created a genomic library that contains environmental insert DNA, and the host is E.coli. The library is made of fosmids, and the copy number is kept at one. I was planning on using colony pcr to screen the inserts present in the library. I was wondering if its possible to use
gram-positive pcr primers to screen the library, so as to avoid the host E.coli DNA, and still be able to screen for a large number of bacterial 16S genes. If anyone with expierience in these techniques could shed some light, it would be appreciated
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