Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primer sequences


  • Please log in to reply
1 reply to this topic

#1 chaperones

chaperones

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 16 May 2011 - 01:32 PM

Hi,
does anyone know how to get primer sequences for reference gene e.g. HPRT or GAPDH which are designed by Roche for experiment? Because when we use Roche UPL we have only sequences for primers of our genes without any information about reference primers and replicon.
Thanks!

#2 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,206 posts
110
Excellent

Posted 17 May 2011 - 12:40 AM

When you design a UPL assay, check Design multiplex PCR with reference gene.
Then your assays would be displayed together with a housekeeping gene, click more multiplex experiments on the bottom to display all references and then click on the "i" in a circle beside each housekeeping - Information about...

You won't however be able to get the probe number or sequence, only primer sequences and amplicon info.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.