Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Why do cells need to be washed in PBS before biotinylation?


  • Please log in to reply
3 replies to this topic

#1 maddi

maddi

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 May 2011 - 07:59 AM

Hi guys,

I was following a procedure for detecting cell surface labeled proteins today in the lab and it required I wash the cells in PBS prior to adding sulfo-NHS-biotin to the tubes containing the cells first. Why is this? The lab manual mentioned that PBS is an isotonic buffer of salts, could it be something to do with charge?

Some insight would be lovely :)

#2 Biouday

Biouday

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 47 posts
28
Excellent

Posted 18 May 2011 - 10:09 PM

As you mentioned you are using PBS just to wash the cells from cell culture medium. It has nothing do with the charge.

#3 mdfenko

mdfenko

    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,369 posts
217
Excellent

Posted 19 May 2011 - 06:43 AM

see this wikipedia page for the meaning of isotonic

talent does what it can
genius does what it must
i used to do what i got paid to do


#4 Gangwolf

Gangwolf

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 41 posts
1
Neutral

Posted 09 September 2011 - 08:47 AM

Probably to get rid of the serum proteins in your cell culture medium.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.