Dear all,
I tried to find answer of the questions I have in textbooks and review-papers, but I could not.
My questions are:
1. I know that the basal lamina is the internal compound of basement membrane - Sanes J.R., J Biol Chem, 2003, 278(15): 12601-12604. What is the origin of the basal lamina - the surrounding interstitial connective tissue (which should be the endomysium in my understanding) or the skeletal muscle fiber?
2. This one is probably stupid, but - do the vascular and lymphatic vessels open into the space between Basal lamina and the sarcolemma, i.e. Lamina lucida? I think they should not, but I am not sure and need someone to confirm.
3. Can satellite cells be recognized using light microscope? I think I see some smaller nuclei that differ to the skeletal muscle nuclei, but I am not a morphologist at all, so that could be a product of my imagination.
Thank you,
Nephrite
0
Three questions about skeletal muscle tissue
Started by Nephrite, May 16 2011 01:51 AM
2 replies to this topic
#2
LyleBabcock
-
- Active Members
-
- 10 posts
member
0
Neutral
Neutral
Posted 23 May 2011 - 09:10 AM
Nephrit, on 16 May 2011 - 01:51 AM, said:
Dear all,
I tried to find answer of the questions I have in textbooks and review-papers, but I could not.
My questions are:
1. I know that the basal lamina is the internal compound of basement membrane - Sanes J.R., J Biol Chem, 2003, 278(15): 12601-12604. What is the origin of the basal lamina - the surrounding interstitial connective tissue (which should be the endomysium in my understanding) or the skeletal muscle fiber?
2. This one is probably stupid, but - do the vascular and lymphatic vessels open into the space between Basal lamina and the sarcolemma, i.e. Lamina lucida? I think they should not, but I am not sure and need someone to confirm.
3. Can satellite cells be recognized using light microscope? I think I see some smaller nuclei that differ to the skeletal muscle nuclei, but I am not a morphologist at all, so that could be a product of my imagination.
Thank you,
Nephrite
I tried to find answer of the questions I have in textbooks and review-papers, but I could not.
My questions are:
1. I know that the basal lamina is the internal compound of basement membrane - Sanes J.R., J Biol Chem, 2003, 278(15): 12601-12604. What is the origin of the basal lamina - the surrounding interstitial connective tissue (which should be the endomysium in my understanding) or the skeletal muscle fiber?
2. This one is probably stupid, but - do the vascular and lymphatic vessels open into the space between Basal lamina and the sarcolemma, i.e. Lamina lucida? I think they should not, but I am not sure and need someone to confirm.
3. Can satellite cells be recognized using light microscope? I think I see some smaller nuclei that differ to the skeletal muscle nuclei, but I am not a morphologist at all, so that could be a product of my imagination.
Thank you,
Nephrite
To answer numbver 3, yes satellite cells can be recognized, especially if you are staining for DNA content, such as a DAPI stain
#3
DLY
-
- Active Members
-
- 7 posts
member
0
Neutral
Neutral
Posted 16 June 2011 - 07:54 AM
LyleBabcock, on 23 May 2011 - 09:10 AM, said:
Nephrit, on 16 May 2011 - 01:51 AM, said:
Dear all,
I tried to find answer of the questions I have in textbooks and review-papers, but I could not.
My questions are:
1. I know that the basal lamina is the internal compound of basement membrane - Sanes J.R., J Biol Chem, 2003, 278(15): 12601-12604. What is the origin of the basal lamina - the surrounding interstitial connective tissue (which should be the endomysium in my understanding) or the skeletal muscle fiber?
2. This one is probably stupid, but - do the vascular and lymphatic vessels open into the space between Basal lamina and the sarcolemma, i.e. Lamina lucida? I think they should not, but I am not sure and need someone to confirm.
3. Can satellite cells be recognized using light microscope? I think I see some smaller nuclei that differ to the skeletal muscle nuclei, but I am not a morphologist at all, so that could be a product of my imagination.
Thank you,
Nephrite
I tried to find answer of the questions I have in textbooks and review-papers, but I could not.
My questions are:
1. I know that the basal lamina is the internal compound of basement membrane - Sanes J.R., J Biol Chem, 2003, 278(15): 12601-12604. What is the origin of the basal lamina - the surrounding interstitial connective tissue (which should be the endomysium in my understanding) or the skeletal muscle fiber?
2. This one is probably stupid, but - do the vascular and lymphatic vessels open into the space between Basal lamina and the sarcolemma, i.e. Lamina lucida? I think they should not, but I am not sure and need someone to confirm.
3. Can satellite cells be recognized using light microscope? I think I see some smaller nuclei that differ to the skeletal muscle nuclei, but I am not a morphologist at all, so that could be a product of my imagination.
Thank you,
Nephrite
To answer numbver 3, yes satellite cells can be recognized, especially if you are staining for DNA content, such as a DAPI stain
