Low sample number array...?
Posted 16 May 2011 - 01:43 AM
I was wondering if there is something like an array-based system in which you can measure the expression of a few genes (lets say 50 genes) in a few samples. I know that for this purpose a qPCR-like technique would be suitable, but I would like an array-based approach.
Does anyone know if something like this exists?
Posted 16 May 2011 - 08:22 AM
genius does what it must
i do what i get paid to do
Posted 18 May 2011 - 06:30 AM
As mdfenko suggests, you could get a custom array made, but this could be a lot of trouble for just a few samples. There are academic labs and core facilities that will print custom arrays much cheaper than commercial producers. Since you are only looking at 50 genes, you could have them print multiple mini arrays on a slide, and you could even print multiple mini arrays in a well-format like these:
from the Gel Company. This allows you do to more samples per chip.
Commercial producers that you may want to consider are Applied Microarrays and MYcroarray. An alternate is CustomArray; their semiconductor arrays are designed for customization and can be read on standard array scanners.
Other technologies to consider are the Luminex bead assay and NanoString Tech., but these require access to specialized hardware.
Posted 23 May 2011 - 01:30 AM