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Non reducing protein ladder?


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5 replies to this topic

#1 avalon

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Posted 15 May 2011 - 12:49 PM

Hi all,

Can anyone recommend a non-reducing protein ladder?

#2 mdfenko

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Posted 16 May 2011 - 10:50 AM

ge healthcare sells a couple of sets of markers that may be what you want (did you mean non-denatured?).

ge healthcare protein standards

protein and peptide mw markers

(the lmw-sds markers do not contain sds or dtt or 2-me, they can be used for native page)

Edited by mdfenko, 16 May 2011 - 10:52 AM.

talent does what it can
genius does what it must
i do what i get paid to do

#3 avalon

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Posted 16 May 2011 - 07:11 PM

ge healthcare sells a couple of sets of markers that may be what you want (did you mean non-denatured?).

ge healthcare protein standards

protein and peptide mw markers

(the lmw-sds markers do not contain sds or dtt or 2-me, they can be used for native page)


Thanks for replying mdfenko, I actually wont be running native gels ( I will be doing sds page), but am trying to detect a biotinylated protein after IP with a specific antibody.....just trying to confirm specificity of my IP without using radioactivity. I havent had much success so far and someone pointed out that the reducing agents in my ladder were probably cleaving the biotin off the protein (I should have used a different biotin to start with). So I guess I just need a ladder without DTT or BME really but a lot of the companies dont seem to specify whether these are present or not.

#4 mdfenko

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Posted 17 May 2011 - 07:16 AM

the lmw standard does not contain reducing agent and is good for sds-page.
talent does what it can
genius does what it must
i do what i get paid to do

#5 avalon

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Posted 17 May 2011 - 12:16 PM

the lmw standard does not contain reducing agent and is good for sds-page.


Thank you!

#6 Jennifer415

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Posted 23 June 2011 - 11:45 AM

Why do you care if your ladder is reduced or not............it runs separately from the other protein lanes! doubtful that the small amount of reducing agent is cleaving biotin in your protein lanes.




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