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Nuclear or Cytoplasmic RNA Purification


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#1 thegene

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Posted 15 May 2011 - 11:18 AM

Dear colleagues,

Anybody know any kit for purifying RNA from the cytoplasm and the nucleus from animal tissue?

I found a kit from Fisher but they did not guarantee that the nuclear fraction will be free of cytoplasmic RNA contamination.
Please, if anyone knows a kit or recommend someway. i will be really thankful.



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#2 pcrman

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Posted 17 May 2011 - 07:51 PM

For RNA isolation from both compartments, there is always the concern of mutual contamination. We had the same problem with a kit from Ambion.




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