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Cells dying after culturing-lowRNA

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#1 gromlik



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Posted 12 May 2011 - 11:38 PM

Please help! We began to perform chondrogenic differentiation from MSC. I centrifuge cells at 400g (from PBS) and count them. At day O everything is OK, at day 7-28, they become more blue (dying). And as aa result low RNA and bad RQ-PCR amplification on days 7-28. How do You think - is it question with cell viability, centrifuging, hands ???

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