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reprogramming of cells


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#1 hrltwc

hrltwc

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Posted 12 May 2011 - 07:25 PM

Hi all,

I;ve been trying to reporgram cells using lentiviruses.. my 293 cells usually turn about 80% green (GFP) after 48 hours.. but when i use the virus to infect cells, i do not get ips, or the ips that I get is usually fake colonies.

Another problem that I am facing is the iPS generated lifts off the surface of the dishes, so I am unable to use the cut and paste method to passage my cells.. anyone encountered this before?




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