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Gene copy number analysis

Started by hemomol, May 12 2011 03:14 PM

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#1 hemomol

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Posted 12 May 2011 - 03:14 PM

Does anybody have any ideas about protocols for gene copy number analysis using Real time qPCR ? Suggestions for determining gene copy number by any other method/techniques are also welcome. Thanks.

#2 Rsm

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Posted 12 May 2011 - 11:19 PM

For determination of inserted gene copy number in transgenic mouse you'd use southern hybridization and count the bands or use densitometry.
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