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MTT different from thymidine

Started by michalcn, May 12 2011 11:50 AM

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#1 michalcn

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Posted 12 May 2011 - 11:50 AM

Hi,
I did proliferation assay that showed decrease in thymidine intake but in the MTT I got no change
what does it means?
thanks
Michal

#2 klinmed

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Posted 06 June 2011 - 03:54 AM

View Postmichalcn, on 12 May 2011 - 11:50 AM, said:

Hi,
I did proliferation assay that showed decrease in thymidine intake but in the MTT I got no change
what does it means?
thanks
Michal
Thymidine incorporation reflects nucleic acid synthesis whilst MTT metabolic activity. Your cell may have stopped synthesizing NA but still be alive and metabolically active.
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