smears in PCR products (bisfulite treated DNA)
Posted 12 May 2011 - 11:15 AM
I've successfully amplified my products with:
Zymo research's "human methylated DNA".
However, when I extract DNA from cell culture, I don't see any products- only smears. I've designed my primers using methylprimer express.
I do 40 cycles.
What does this mean?
Posted 15 May 2011 - 08:56 AM
Posted 17 May 2011 - 07:36 AM
I've tried that but no luck. Only smears...