Cell cycle evaluation in cells with more than one nuclei
Posted 12 May 2011 - 01:22 AM
I want to measure the distribution of the cell cycle in my cells. But I have a big problem. My cells have more than one nuclei (usually 4-5) and the cell cycle is asynchronous. Therefore, within the same cell the different nuclei are in different phase of the cycle.
So, I can't use the traditional propidium iodide staining of DNA/flow cytometry to analyse this. Can anyone indicate me an alternative method for this?
Posted 12 May 2011 - 08:37 PM
Out of curiosity: how do you know the difference nuclei are in different stages of the cell cycle?
Posted 13 May 2011 - 12:52 AM
I think I could synchronize the cells by chemical means. But then how would I evaluate the effects of drugs in cell cycle distribution?
Regarding the other question, I simply looked in the literature and saw that these nuclei divide asynchronously.
Posted 13 May 2011 - 04:26 PM
You could make the assumption that each individual nucleus is a "cell" and work from there?
Thanks for the info re the nuclei, that's pretty interesting... I wonder how it works for cell division?
Edited by bob1, 13 May 2011 - 04:27 PM.
Posted 14 May 2011 - 07:36 AM
What do you mean by "switch to mononucleate cells"? Change the type of cells I am working with? That is not possible, because I am working with a fungus.
Yes, it is interesting how the cells can orchestrate asynchronous cell division.
Posted 15 May 2011 - 03:42 PM
I have just realised that treating each nucleus as a cell may not work either - they may be too close together to measure the different nuclei, and you certainly wouldn't be able to cell sort, if you were aiming for that.
Posted 16 May 2011 - 01:16 AM
Do you have any other suggestion to evaluate cell cycle?
Posted 16 May 2011 - 06:37 PM