1 reply to this topic

[krisw]

Hello everyone,

I have been purchasing high quality RNA from Ambion to construct my standard curves with. (I am running relative standard curve to test for gene expression.) The main problem is that I am using a ton of this very expensive RNA. Is there a better alternative? Can I use my reference sample for the standard curve?

Thanks,
Kris

[Trof]

You can use any RNA that is concentrated enough to fill range of your samples when serially diluted. But if you use efficiency corrected relative quantification formula instead of standard curve method, you only need to run the standard curve once to get the efficiency.