What to use for Standard Curve
Posted 11 May 2011 - 10:29 AM
I have been purchasing high quality RNA from Ambion to construct my standard curves with. (I am running relative standard curve to test for gene expression.) The main problem is that I am using a ton of this very expensive RNA. Is there a better alternative? Can I use my reference sample for the standard curve?
Posted 12 May 2011 - 12:29 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 25 May 2013 - 06:13 AM
could you please suggest any papers that using the efficiency corrected relative quantification formula? Or could you please explain a bit about that?
I've read the qiagen protocol for real-time pcr kit (critical factors for successful real time pcr), it stated that if there are differences in PCR efficiency between the HK gene and GOI gene, the Livak and Pfaffl methods cannot be used, so we have to generate the standard curve. So if the pcr efficiency is not comparable, can I use that "efficiency corrected relative quantification formula"? Or is that formula is one of Livak or Pfaffl methods?