Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Help! Transfection newbie with a dumb question

  • Please log in to reply
1 reply to this topic

#1 slowlearner



  • Members
  • Pip
  • 1 posts

Posted 11 May 2011 - 03:12 AM

I apologize if this is a stupid question but I really don't understand.

I want to transfect my adherent cell line with a plasmid using FuGENE 6. I've downloaded the protocol/info from Roche on FuGENE and have read it but I am so lost. After completing the actual protocol, how do I determine whether my cells were actually transfected? The Roche pdf simply says to measure reporter gene activity -- how do I do that? Is there a kit or something I can buy?

#2 knuf



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 104 posts

Posted 11 May 2011 - 06:22 AM

Generally this is done by either western blot or immunocytochemistry and measure the protein expression.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.