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Help! Transfection newbie with a dumb question


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#1 slowlearner

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Posted 11 May 2011 - 03:12 AM

I apologize if this is a stupid question but I really don't understand.

I want to transfect my adherent cell line with a plasmid using FuGENE 6. I've downloaded the protocol/info from Roche on FuGENE and have read it but I am so lost. After completing the actual protocol, how do I determine whether my cells were actually transfected? The Roche pdf simply says to measure reporter gene activity -- how do I do that? Is there a kit or something I can buy?

#2 knuf

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Posted 11 May 2011 - 06:22 AM

Generally this is done by either western blot or immunocytochemistry and measure the protein expression.




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