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How to blast methylation specific and unspecific primers


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#1 jylow85

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Posted 10 May 2011 - 11:41 PM

Dear all, need you kind assitance to help me on how can I blast the methylation specific and unspecific primers to see which position it binds to the DNA.

Thanks.

#2 pcrman

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Posted 15 May 2011 - 08:53 AM

To be able to do that, you need a bisulfite modified genome as the reference sequence. It seems BiSearch provides such function.




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