ELISA standard curve calculation question
Posted 10 May 2011 - 06:27 PM
Posted 13 May 2011 - 07:29 AM
Posted 03 June 2011 - 03:39 PM
I got one protocol on www.novateinbio.com ( ELISA kit instruction URL: http://www.novateinb...instruction.pdf)
It has detailed instruction on how to draw standard curve and get the formula with Excel, and then use the formula to calculate the analyte concentration from OD450. Take a look. It may help.