Salvaging contaminated Matrigel
Posted 10 May 2011 - 06:04 PM
I am suspecting some of my small Matrigel aliquots are contaminated. I observed the Matrigel-coated filter under the microscope and none of my cells invaded through the membrane. I can only see bacteria-like stuffs which makes me worry a lot.
If my small Matrigel aliquots are really contaminated, is there any way to salvage it? Is it possible to add serum-free medium supplemented with antibiotic while diluting it to working concentration? Please help as I do not really want to discard them straight away. I would greatly appreciate if there are good tips and ideas other than discarding them.
Thank you very much and warmest regards,
Posted 10 May 2011 - 07:06 PM
Posted 10 May 2011 - 07:36 PM
Posted 11 May 2011 - 05:02 PM
Posted 11 May 2011 - 06:39 PM
Yesterday I tested one of the small Matrigel aliquots for contamination. I diluted the Matrigel with serum-free medium and cultured it overnight. This morning when i took from the incubator to observe, there was no sign of contaminants =). But to be on safe side, I will leave it for another day.
Bob1, have you ever had an experience whereby your Matrigel coat formed a "basin" when it is dry? The gel concentrates and mainly dries on the periphery side of the membrane rather than the centre. As a result, the coating of the membrane was uneven and the cells tend to invade across the membrane centre. Do you have any recommendation or ideas to solve this problem? Thank you very much
Posted 12 May 2011 - 08:35 PM