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Cell Senescence marker for histology


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#1 Rnotk

Rnotk

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Posted 10 May 2011 - 04:20 PM

Hi all,

I am currently looking for some antibodies for cell senescence.
I did some literature search, and thinking to test either SMP30, p21, MRF4 or p16INK4a antibody.

but when I look the abs companies, not sure which is better than the other.
and I know it is normally hard to find "good" antibody until you tested by yourself.

So, I am asking anyone have any experience in these antibodies????
if you have any antibodies that you can recommend, or not recommend, please let me know.

I already asked few of the labs in our institution, but no one study senescence here, so I have no choice but purchasing.

Also, I realized many of the senescence antibody is anti-human, but I am looking for Anti-mouse antibody for mouse tissue (frozen tissue), either IFC or IHC.

any suggestion helps
BTW, I know the SA-beta gal activity, and I cannot do this to my tissue due to some other reason.

Thanks in advance




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