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Growth curve for PC3 cell line


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#1 Huda

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Posted 10 May 2011 - 08:04 AM

Hi
I am doing a growth curve in 24 well plate starting with 5000 cell/ml in each well and counting very 2 days for 12 day since the PC3 cell are cancer cell so the growth curve should be sigmoidal since they usually don't die but I have reached day 10 and they are still growing in very high rate so I could not see the day where they have maximum growth yet what did I do wrong ?????

#2 gfischer

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Posted 10 May 2011 - 09:04 AM

Cancer cells often don't have contact inhibition, and consequently will not stop dividing even when they have reached confluence. You may not be able to get a true sigmoidal curve.
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