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[Huda]

Hi
I am doing a growth curve in 24 well plate starting with 5000 cell/ml in each well and counting very 2 days for 12 day since the PC3 cell are cancer cell so the growth curve should be sigmoidal since they usually don't die but I have reached day 10 and they are still growing in very high rate so I could not see the day where they have maximum growth yet what did I do wrong ?????

[gfischer]

Cancer cells often don't have contact inhibition, and consequently will not stop dividing even when they have reached confluence.  You may not be able to get a true sigmoidal curve.