During my nuclear lysis, I add 1ul of NP-40 from the bottle to each lysate to rupture the membrane. Then after spinning it down, the supernatant is collected as the cytosolic fraction. The last couple of times I have done this, I've had sticky insoluble pellets and I want to avoid this. Will I get away with diluting down the NP-40 to say 10% in my lysis buffer so it performs the same function and thus I avoid this globby pellet. Also, if I do this, how much should I add (v/v)?
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