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miRNA microarray using TLDA


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#1 banou

banou

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Posted 10 May 2011 - 03:44 AM

Hi,
Have you ever done Taq-man Low Density Array Cards (TLDA)for miRNA. I don't know whether the fractionation of small RNAs is necessary. (for cDNA synthesis step) I am afraid of losing some low abundant miRNAs in case of using total RNA for cDNA synthesis.
Thanks

Edited by banou, 10 May 2011 - 03:46 AM.


#2 banou

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Posted 13 May 2011 - 03:51 AM

Dear all interested scientists!
I asked from a group who already published a paper with this technique; and they wrote me back that you have to use Total RNA not fractoned small RNA.
Thanks,
Banou

Edited by banou, 13 May 2011 - 03:52 AM.





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