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phosphorylted protein smaller not bigger?


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#1 sBrow

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Posted 10 May 2011 - 03:24 AM

Hi
I am researching and re researching Sirt1 deacetylase
It appears that the protein, when phosphorylated, at least for mouse, runs further on SDS gel. can anyone shed light on this?
thanks
Suse

Edited by sBrow, 10 May 2011 - 03:24 AM.


#2 mdfenko

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Posted 10 May 2011 - 10:05 AM

the incorporated phosphate(s) can increase mobility, it increases negative charge on the protein. we used urea gels to visualize phosphorylated vs unphosphorylated proteins and the phosphorylated ran faster (urea enhances charge separations).

Edited by mdfenko, 10 May 2011 - 10:06 AM.

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