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Agrobacterium EHA101 competent cells


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#1 charisma

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Posted 10 May 2011 - 01:21 AM

Hi,

Could u plz help me out to prepare EHA101 competent cells. I wonder what antibiotics should I add in the LB plate for strain EHA101?
Rif?Kan?

Cheers,
Charisma

#2 pito

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Posted 10 May 2011 - 01:29 AM

Isnt EHA101 kanamycine resistant?

Anyway: if you have some antibiotics, why not just test it? Plate some out and see if they grow?

Does it still contain pIG121-Hm if so, Hygromycin B can be used if I remember correctly.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.





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