Posted 10 May 2011 - 12:31 AM
I had a question about the quantification of DNA using the Nanodrop,
I amplified my DNA using normal PCR amplification i purified it with a Qiaquick PCR purification kit and then i measured my samples using the nanodrop, i had the following results:
Concentration (ng/ul) = 18,0
260/280 nm = 1,78
260/230 nm = 2,88!! <- This value concerns me
Does anyone of you have an idea what this high value means, i have looked up some forums and they said it was because the concentration of the DNA was too low but i don't think that this is the problem.
Fact about the DNA molecule, the 5' strand of the DNA molecule has a NH2-C6 group attached to it, could it possibly be some kind of absorbance either from the NH2 or the C6 chain?
Can anyone help me?
Posted 10 May 2011 - 02:44 AM
Posted 10 May 2011 - 12:56 PM