THP-1 cell line
Posted 09 May 2011 - 11:21 PM
Kindly let me know what should I do so that the cell start to grow. I need them for my experiment.
Posted 11 May 2011 - 04:53 AM
What protocol for defrosting are you currently using?
Posted 11 May 2011 - 06:25 AM
When I take the vial out I let it 1/2 thaw in a water bath and then transfer it to 4 mL of recovery media in Recovery Media(RPMI media, serum, antibiotics and extra L-glutamine) and centrifuge it at 100xg for 5 minutes. Resuspend it in 8mL of the recovery media and transfer it to a T-25. I let this sit in the incubator (37C, 5%CO2) for a week checking cell count and morphology every other day. Passage your cells at least once after this before using them.
Hope this helps! If the cells were poorly preserved this could be a major factor in their inability to recover and grow.
Edited by Ricey, 11 May 2011 - 06:25 AM.