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Inducing apoptosis in ESCs by UV


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#1 PKNubbins

PKNubbins

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Posted 09 May 2011 - 09:03 AM

So I need a positive control for western blot to show that some cleaved caspase antibodies (3,9) are working correctly. Since we grow ESCs in our lab regularly, it seems to me that UVing some in our stratalinker would be a quick and easy way to make this positive control..

Anyone have any experience inducing apoptosis in ES cells, in terms of how much UV exposure to give and how long to wait afterwards? I was thinking ballpark 50J/m2, followed by overnight 12-16 hour incubation..




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