will my phosphorylated GST tagged peptide stay phosphorylated if stored in regular denaturing sample buffer overnight before running the gel or is it better to run the gel immediately?
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#2
knuf
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Posted 10 May 2011 - 09:08 AM
Assuming that it is phosphorylated to begin with--ie, you added phosphatase inhibitors and what not to your lysis buffer--it should be fine to run the next day
#3
Inmost sun
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Posted 17 May 2011 - 04:56 AM
in general, it is not recommended to store proteins in sample buffer; but if, it would be useful to store in a phosphate containing buffer which, however, is rarely found in sample buffers
