Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

protein phosphorylation status in sample buffer


  • Please log in to reply
2 replies to this topic

#1 xenopus 1

xenopus 1

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 09 May 2011 - 08:47 AM

will my phosphorylated GST tagged peptide stay phosphorylated if stored in regular denaturing sample buffer overnight before running the gel or is it better to run the gel immediately?

#2 knuf

knuf

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 104 posts
3
Neutral

Posted 10 May 2011 - 09:08 AM

Assuming that it is phosphorylated to begin with--ie, you added phosphatase inhibitors and what not to your lysis buffer--it should be fine to run the next day

#3 Inmost sun

Inmost sun

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 303 posts
60
Excellent

Posted 17 May 2011 - 04:56 AM

in general, it is not recommended to store proteins in sample buffer; but if, it would be useful to store in a phosphate containing buffer which, however, is rarely found in sample buffers




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.