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protein phosphorylation status in sample buffer
2 replies to this topic
Posted 09 May 2011 - 08:47 AM
will my phosphorylated GST tagged peptide stay phosphorylated if stored in regular denaturing sample buffer overnight before running the gel or is it better to run the gel immediately?
Posted 10 May 2011 - 09:08 AM
Assuming that it is phosphorylated to begin with--ie, you added phosphatase inhibitors and what not to your lysis buffer--it should be fine to run the next day