3 replies to this topic

[cellgene]

Dear All,

The question may sound quite stupid, but a colleague of mine and me calculated several times and we cannot agree on this calculation. Therefore I would like to ask you if this calculation is correct.

We have a 2.5mM solution of a protein that is 25.9kDa.

25.9kDa=25900 g/mol = 25900 mg/mmol
2.5mM = 2.5 mmol/l

2.5*25900=64750 mg/l
so it is 64.75 mg/ml

do you agree?

thanks a lot for your help!

[mdfenko]

looks right to me

what does your colleague say?

Edited by mdfenko, 09 May 2011 - 07:01 AM.

[cellgene]

mdfenko, on 09 May 2011 - 07:00 AM, said:

looks right to me

what does your colleague say?

thanks for the answer
she calculated 64,75 micrograms/ml  

[Prep!]

ur answer is correct cellgene... i calculate like this

25900g - 1000mM
  ?    -  2.5mM

64.75g - 2.5 mM - 1000 ml
  ?    - 2.5 mM - 1ml

Answer - 64.75 mg