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calculation from Molar to g/ml


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3 replies to this topic

#1 cellgene

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Posted 09 May 2011 - 06:49 AM

Dear All,

The question may sound quite stupid, but a colleague of mine and me calculated several times and we cannot agree on this calculation. Therefore I would like to ask you if this calculation is correct.

We have a 2.5mM solution of a protein that is 25.9kDa.

25.9kDa=25900 g/mol = 25900 mg/mmol
2.5mM = 2.5 mmol/l

2.5*25900=64750 mg/l
so it is 64.75 mg/ml

do you agree?

thanks a lot for your help!

#2 mdfenko

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Posted 09 May 2011 - 07:00 AM

looks right to me

what does your colleague say?

Edited by mdfenko, 09 May 2011 - 07:01 AM.

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#3 cellgene

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Posted 09 May 2011 - 07:07 AM

looks right to me

what does your colleague say?

thanks for the answer
she calculated 64,75 micrograms/ml :huh:

#4 Prep!

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Posted 09 May 2011 - 08:02 PM

ur answer is correct cellgene... i calculate like this

25900g - 1000mM
? - 2.5mM

64.75g - 2.5 mM - 1000 ml
? - 2.5 mM - 1ml

Answer - 64.75 mg

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