Posted 08 May 2011 - 07:28 PM
am looking for a protein of nearly 35 kDa via Western blot for which i use specific antibody...this time when i performed western, apart from this band (which was weak) i saw a dark band of about 60 kDa..the antibody is said to be specific...does anyone know how to account for this?
Posted 09 May 2011 - 08:12 AM
genius does what it must
i do what i get paid to do
Posted 09 May 2011 - 07:57 PM
Posted 23 May 2011 - 02:28 PM
could be an artifact which shows up when using aged 2-mercaptoethanol (and dust). it will stain non-specifically.
thnx for the reply but i have bis tris gel and pvdf membrane, no silver..will it be an artifact?