RNA extraction. Accidentally adding isopropanol into trizol(with tissues) instead of chloroform.
Any chance I can rescue my samples?
Millions of thanks
Accidentally add isopropanol into Trizol
1 reply to this topic
Posted 08 May 2011 - 12:06 AM
Hmmm, not sure on this... I think the RNA will still be there, though it should have precipitated out and will be heavily contaminated with phenol and proteins. You could try just adding chloroform to get rid of the trizol and go from there, like you would if you were cleaning up a RNA sample for phenol contamination.