how to check my primers from Roche UPL by using BLAST?
e.g. for XYLT1 gene, Roche UPL designed for me these primers: L GTTGCGCCTCTGACCTCCT and R CAGCTTCAGTGCCTCCTCA with amplicon 60bp,I wrote this sequences to BLASTN but Im not sure hot to interpret results.
primers are for cDNA
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