Dear all,
I have finshed my microarray experiments and I'd like to validate around 20 genes in four samples through RT-PCR SYBR Green.
I am using StepOnePlus ABI, and B2M gene for normalization.
Just simple questions, do I need to run B2M relative standard curve for every run or just do it one time and do the normalization manually? Is this acceptable for publications or they would ask to run the experiments under the same condition?
Thanks in advance.
Best regards
Salem
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