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excel and propagation of error, qpcr


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#1 thumbclaw

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Posted 06 May 2011 - 08:48 AM

Hi all,

I'm no mathematician, but I know that the error from my control set and from my experimental set should be reflected in final error bars.

1. What is the appropriate formula to use (there seems to be more than one way to look at this)
2. How do you tell excel to do it?

Thanks for any help.

#2 tea-test

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Posted 06 May 2011 - 12:49 PM

all the necessary formulas for the proper error propagation can be found in this paper:

Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J. qBase relative
quantification framework and software for management and automated analysis of
real-time quantitative PCR data. Genome Biol. 2007;8(2):R19. PubMed PMID:
17291332

full text is free.
tea-test: The artist formerly known as Ned Land




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